100bp DNA Ladder 100-700 bp for Gel Electrophoresis

Product Information：

The YB100bp DNA Ladder, consists of 7 linear double-stranded DNA (containing 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, and 700 bp) bands, which have been mixed with 1×DNA loading buffer containing blue indicator dye, and is suitable for the analysis of DNA bands in agarose gel electrophoresis. This product is ready-to-use, according to the experimental needs, take 5-10 μL electrophoresis, easy to use, clear electrophoresis bands, easy to accurately determine the content of the target product DNA. The 500 bp band in YB100bp DNA Ladder has a DNA concentration of 100 ng/5 μL and shows a bright band; the remaining bands have a DNA concentration of 50 ng/5 μL.

Storage Conditions：

Ship with wet ice; Store at -20℃, valid for 24 months.

Instructions for Use:

1. After thorough thawing and mixing, take 5 μL of the product and add it to the sampling wells of the agarose gel (the volume of the sample volume is proportional to the width of the wells: 1 μL: 1 mm, if the sampling wells are wider, the volume of the sample volume can be increased appropriately), and then carry out electrophoresis.

2. Recommended electrophoretic conditions are 2% agarose (Y101411-100g recommended) gel at 4-10 V/cm.

3. Stain by EB or other nucleic acid dyes (Y101421-100UL recommended) and observe the electrophoretic bands under a UV lamp or gel imager.

Mix gently, and then load 1 μl per 1 mm gel lane.

Notice: 

1. When using, thaw and mix thoroughly, thaw and store at 4℃, avoid freeze - thaw cycles.

2. Change the electrophoresis buffer and use freshly prepared agarose gel in time to avoid affecting the electrophoresis results.

3. For your safty and health,please wear safety glasses, gloves, or protective clothing.
4. For Reseacrch Use Only