Antibody Stripping Buffer Normal Level

Product Information：

Antibody Stripping Buffer is a reagent that safely and efficiently removes primary and secondary antibodies from nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes, facilitating the re-detection of chemiluminescent protein immunoblots.

Save time and effort, eliminate errors: No need to re-gel, sample and electrophoresis; save precious samples; eliminate re-sampling errors.

Quick and efficient: Incubate for 5-15 minutes at room temperature to strip antibody.

Mild formulation: Does not interfere with protein re-assay after elution.

Odorless: Thiol-free, no offensive odors.

Ready and easy to use: No dilution necessary, ready to use with open cap.

Storage Conditions：

Ship with wet ice, store at 2-8℃, valid for 12 months.

Instructions for Use:

1. Wash blot once for 5 minutes in TBST to remove the chemiluminescent substrate.

2. Pour off the TBST, add sufficient Antibody Stripping Buffer to cover the NC or PVDF membranes and incubate for 5 to 15 minutes at room temperature with gentle shaking.

3. Remove the blot from the Antibody Stripping Buffer and wash 3 times for 5 minutes each in TBST.

4. Follow-up work such as blocking and antibody incubation is performed according to the purpose of the experiment.

Notice: 

1. This product is corrosive, take caution when using it.

2. Reuse is not recommended.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.